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Functional isoDNA aptamers: modified thrombin binding aptamers with a 2[prime or minute]-5[prime or minute]-linked sugar-phosphate backbone (isoTBA)

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Chem. Commun., 2014, Advance Article
DOI: 10.1039/C3CC47569G, Communication
Anita D. Gunjal, Moneesha Fernandes, Namrata Erande, P. R. Rajamohanan, Vaijayanti A. Kumar
An isomeric 2[prime or minute]-5[prime or minute]-DNA aptamer (isoTBA) of the SELEX-derived thrombin binding DNA aptamer (TBA) forms a quadruplex fold with a chaperone-like activity of the large protein thrombin, or in the presence of cations such as K+/Na+. Its activity in the process of blood clot formation was also confirmed.
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