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In Vitro Monitoring of the Formation of Pentamers from the Monomer of GST Fused HPV 16 L1

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Chem. Commun., 2013, Accepted Manuscript
DOI: 10.1039/C3CC44986F, Communication
Dongdong Zheng, Dong Pan, Xiao Zha, Yuqing Wu, Chunlai Jiang, Xianghui Yu
The recombinant GST fusion protein HPV 16 L1 from E. coli was proved to exist as the monomer rather than the pentamer, supplying the possibility of real-time monitoring of the pentamer formation in vitro. Time-dependent kinetics studies of the process were performed for the first time by using static light scattering and western blot, where the essential factors were revealed, offering a new biotechnical way for virus control and/or the development of anti-virus agents.
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